Evidence of Bombyx mori (Lepidoptera: Bombycidae) odorant receptors related to oviposition behavior

Chanikarn Navakeatpreecha; Hikari Nakagi; Piriya Putanyawiwat; Jutarat Jamkratoke; Banthari Chotimanothum; Anchanee Kubera

doi:10.26789/AEB.2024.02.010

Evidence of Bombyx mori (Lepidoptera: Bombycidae) odorant receptors related to oviposition behavior

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Chanikarn Navakeatpreecha, Hikari Nakagi, Piriya Putanyawiwat, Jutarat Jamkratoke, Banthari Chotimanothum, Anchanee Kubera

Abstract

The silkworm, Bombyx mori, is an insect that is economically important for silk production, cosmetics, medical applications, food, and scientific research. The oviposition behavior of the female moth affects the number of eggs and the volume of silk production. This research aimed to investigate the relationship between the various treatment conditions of mulberry odor, the expression levels of Bombyx mori odorant receptor genes (BmOrs) in the antenna of female Bombyx mori moths, and the moths’ oviposition behavior. Four treatment conditions (fresh mulberry leaves, 2% mix, mulberry leaf juice, and 2% powder) showed a higher oviposition rate than that of the control. Our results revealed that the expression levels of BmOr44, BmOr54, BmOr56, and BmOr63 might play a major role in oviposition. The predicted three-dimensional structures of BmOr44, BmOr54, BmOr56, and BmOr63 proteins were found similar and some active compounds of mulberry leaf could virtually bind to these proteins. The expression patterns of BmOr19 and BmOr30, the specific female adult moth odorant receptor genes, were similar in almost every treatment.

Keywords

Silk moth, olfactory receptors, egg laying

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DOI: https://doi.org/10.26789/AEB.2024.02.010

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